ThinPrep Pap Test: Body Fluid Testing Information from Cytyc

Body Fluids
(Serous Effusions, Urinary and Cerebrospinal Fluids)

	1. Collection Collect body fluids fresh. Urine may be collected into PreservCyt® Solution. A 2:1 urine-to-PreservCyt ratio is required. The use of the ThinPrep® UroCyte Urine Collection Kit is recommended. Fluids collected in CytoLyt® Solution require a CytoLyt Solution Wash prior to instrument processing. For extremely bloody fluids, start with only 10ml of fresh fluid.
	2. Concentrate by Centrifugation (600g for 10 minutes) Note: To reduce the time required for the centrifugation step, operate your centrifuge at 1200g for 5 minutes.
	3. Pour Off Supernatant and Resuspend Cell Pellet Resuspension can be done on a vortexor or may be achieved by syringing the pellet back and forth with a plastic pipette.
	4. CytoLyt Solution Wash Add 30ml CytoLyt Solution Centrifuge Pour Off Supernatant Resuspend Cell Pellet
	5. Evaluate Cell Pellet Appearance If cell pellet is not free of blood, add 30 ml of CytoLyt and repeat from step 2.
	6. Add Specimen to PreservCyt Solution Vial Allow to stand in PreservCyt Solution for 15 minutes.
	7. Run on ThinPrep Processor using Sequence 2 (FLU/FNA) (Fix, stain, and evaluate)

Refer to the ThinPrep Processor Operator's Manual for detailed instrument and specimen preparation instructions.
P/N 85490-002 Rev. I