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Fine Needle Aspirates (FNA)
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1. Collection
Collect sample directly into 30ml of CytoLyt® Solution
CytoLyt Solution is an alcohol-based solution. If a fresh, non-alcohol fixed specimen is clinically indicated, collect the specimen into a balanced electrolyte solution (BES). In the laboratory, the BES is replaced with 30ml of CytoLyt Solution.
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2. Concentrate by Centrifugation
(600g for 10 minutes)
Note: To reduce the time required for the centrifugation step, operate your centrifuge at 1200g for 5 minutes.
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3. Pour Off Supernatant and Resuspend Cell Pellet
Resuspension can be done on a vortexor or may be achieved by syringing the pellet back and forth with a plastic pipette.
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4. Evaluate Cell Pellet Appearance
If cell pellet is not free of blood, add 30ml of CytoLyt Solution and repeat step 2.
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5. Add Specimen to PreservCyt® Solution Vial
Allow to stand in PreservCyt Solution for 15 minutes.
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6. Run on ThinPrep® Processor using Sequence 2 (FLU/FNA)
(Fix, stain, and evaluate)
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Refer to the ThinPrep Processor Operator's Manual for detailed instrument and specimen preparation instructions.
P/N 85489-002 Rev. I
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