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Mucoid Specimens
(Respiratory and Gastrointestinal specimens)
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1. Collection
Sputum:
Collect sample directly into CytoLyt® Solution.
Brushings:
Deposit the collection brush directly into a pre-filled
CytoLyt Solution tube.
Washings/Lavages:
Collect sample with a balanced electrolyte solution
(BES). In the laboratory, the BES is replaced with 30ml of CytoLyt Solution.
The use of DithioThreitol (DTT) has shown to reduce the amount of
mucus present in respiratory samples 1,2. To use DTT with the ThinPrep®
System, prepare a stock solution by adding 2.5g DTT to 30ml of CytoLyt solution. This
solution is suitable for use for 1 week when stored at room temperature (15-30 degrees
C). Add 1ml of stock solution to the sample.
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2. Mechanical Agitation
Place 50ml specimen tubes on the Multi-Mix™ Racked Vortexor for 10
minutes.
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3. Concentrate by Centrifugation
(600g for 10 minutes)
Note: To reduce the time required for the centrifugation step, operate
your centrifuge at 1200g for 5 minutes.
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4. Pour Off Supernatant and Resuspend Cell Pellet
Resuspension can be done on a vortexor or may be achieved by syringing the
pellet back and forth with a plastic pipette.
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5. Evaluate Cell Pellet Appearance
Confirm the cell pellet is in liquid form. If not, add 30ml CytoLyt
Solution and repeat steps 2 and 4.
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6. Add Specimen to PreservCyt® Solution Vial
Allow to stand in PreservCyt Solution for 15 minutes.
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7. Run on ThinPrep Processor using Sequence 3
(Mucoid)
(Fix, stain, and evaluate)
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1 Tockman, MS et al., Safe Separation of Sputum Cells from
Mucoid Glycoprotein, Acta Cytologica 39, 1128 (1995).
2 Tang, CS et al., DithioThreitol Homogenization of Prefixed Sputum for Lung Cancer
Detection, Diagnostic Cytopathology, 10, 76 (1994).
Refer to the ThinPrep Processor Operator's Manual for detailed instrument and specimen
preparation instructions.
P/N 85488-002 Rev. I
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