ThinPrep UroCyte Information for Medical & Lab Professionals

ThinPrep® UroCyte™
(For use with Urine Cytology Processing or Slide-based Molecular Testing)

	1. Collection Collect urine directly into the ThinPrep UroCyte Urine Collection Kit, OR process urine fresh. (Note: Fresh urine can be mixed with a 2:1 urine to PreservCyt® ratio and stored for up to 48 hours before processing.)
	2. Concentrate by Centrifugation (600g for 10 minutes)
	3. Pour Off Supernatant and Resuspend Cell Pellet Resuspension can be done on a vortexor or may be achieved by syringing the pellet back and forth with a plastic pipette.
	4. Add CytoLyt® Solution Wash Add 30ml of CytoLyt Solution Centrifuge Pour Off Supernatant Resuspend Cell Pellet
	5. Evaluate Cell Pellet Appearance If cell pellet is not free of blood, add 30ml of CytoLyt and repeat from step 2. 6. Add Specimen to PreservCyt Solution Vial Allow to stand in PreservCyt Solution for 15 minutes.
	7. Run on ThinPrep Processor using Sequence 5 (UroCyte) (Fix, stain, and evaluate cytology, or perform molecular diagnostic testing according to the manufacturer's instructions for use.)

Refer to the ThinPrep Processor Operator's Manual for detailed instrument and specimen preparation instructions.

ThinPrep UroCyte
Instructions for using the ThinPrep UroCyte Urine Collection Kit

	Note: The specimen collection cup has a blue cap; the PreservCyt vial has a white cap. Step 1) On the specimen collection cup, record patient information in the space provided.
	Step 2) Collect urine in a routine manner. If urine volume exceeds 60cc, pour off excess. The total volume of urine must not exceed 60cc.
	Step 3) After urine is collected, carefully pour PreservCyt into specimen cup containing urine. Do not spill PreservCyt.
	Step 4) Tightly secure blue cap on specimen cup to prevent leakage. (Keep turning for another 1/4 inch after you hear the audible click.)
	Step 5) Place cup and absorbent pads into biohazard bag. Tightly seal bag. Step 6) Store between 4ºC and 30ºC (39ºF-86ºF). Preferred storage and shipping conditions are on ice packs (e.g., blue ice in Styrofoam). Specimen must be processed within 48 hours. Transport the specimen according to your internal procedures.

Please refer to ThinPrep UroCyte PreservCyt Instructions for Use for complete instructions and information on limitations, warning, and precautions associated with the use of PreservCyt.
P/N 86369-002 Rev. C