Brush/Spatula Protocol
Obtain...
...an adequate sampling from the ectocervix using a plastic spatula.
Rinse...
...the spatula as quickly as possible into the PreservCyt® solution vial by swirling the
spatula vigorously in the vial 10 times. Discard the spatula.
Obtain...
...an adequate sampling from the endocervix using an endocervical brush device. Insert the
brush into the cervix until only the bottommost fibers are exposed. Slowly rotate 1/4 or 1/2
turn in one direction. DO NOT OVER-ROTATE.
Rinse...
...the brush as quickly as possible in the PreservCyt solution by rotating the device in the
solution 10 times while pushing against the PreservCyt vial wall. Swirl the brush vigorously
to further release material. Discard the brush.
Tighten...
...the cap so that the torque line on the cap passes the torque line on the vial.
Record...
...the patient's name and ID number on the vial. The patient information and medical
history on the cytology requisition form.
Place...
...the vial and requisition in a specimen bag for transport to the laboratory.
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